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Demystifying CO2

9 min read

Some of the most common questions surrounding localized cryotherapy (such as with the Subzero from America Cryo) are about the gas required for the treatment, CO2. Lets dive into some of the most common questions, shall we?

Why CO2?

People often think that Localized Cryotherapy uses liquid Nitrogen, and while certain older systems as well as full body Cryotherapy Chambers do use Nitrogen, liquid CO2 is much more commonly used in Localized Cryotherapy. Lets compare the two:

What exactly do I need?

So, lets get specific. What exactly do you need to (other than the device, of course!) for Localized Cryotherapy?

Grade of CO2.

There are three different grades of CO2 that are typically available:

While all three grades of CO2 are appropriate for use with localized cryotherapy machines, I recommend looking for Commercial Grade CO2 first. It is normally the easiest to source and least expensive, though in some locations Food Grade CO2 may be more readily available. Praxaire and Linde are common distributors where you can source CO2.

Tanks.

The next thing to consider is what size of tank will work best for you. While there are multiple options, 20 pound and 50 pound tanks tend be best in most circumstances. 20 pound tanks are roughly the size of a scuba tank, and are better if you have to take the treatment to the horse. 50 pound tanks are larger and heavier, and typically better if you can have a treatment area and can bring the horse to the setup. CO2 tanks should normally come with a label clearly showing that they contain CO2.

CO2 Label

One last consideration, your CO2 tank will need a Siphon Tube. Also referred to as a Dip or Eductor Tube. This is essentially a straw that is essentially a straw from the valve on the top of the tank down into the liquid CO2 resting at the bottom of the tank. The liquid CO2 is what allows Localized Cryotherapy to achieve the rapid temperature drop required for the therapy to be effective. Thankfully, getting a tank with a Siphon is just a request you need to make with the supplier when picking up a tank. Tanks with Siphons will typically have a label specifying they are intended for liquid withdrawal.

Liquid Label

Is it safe?

Generally speaking, handling and use of Compressed CO2 is safe to handle under most circumstances. That being said, there are a few items to keep in mind:

Help! I think I’m out of CO2!

There are a few reasons your CO2 tank may be empty, but lets start with the obvious:

You are actually out of CO2!

Of course, the Liquid CO2 in the tank is a limited resource. That being said, there is a little bit of nuance in understanding when a tank is empty. A functionally empty tank will still have pressure, so you can judge based on gas venting from the device. A tank that still has useful Liquid CO2 will discharge a foggy cloud, and a tank that is out of Liquid CO2 will have a primarily clear discharge. The cloud density will vary based on the relative humidity, but it will always be visible.

Comparing Discharge from a full vs empty tank

In most circumstances, you will get slightly less useable CO2 than the, for example, 20 pounds advertised. There are a few reasons for this:

You can keep track of how much CO2 you are getting out of a tank by weighing the tank when its fresh, and again once it presents as empty. The difference between the fresh and empty weight will match the amount of CO2 used. Based on my experience with the Subzero, you will use about a pound of CO2 per minute of discharge with the middle size nozzle.

TankSiphonTubeLiquid CO2Level

A fresh tank immediately appears empty.

There are two reasons a fresh tank can appear empty. Sometimes, the gas supplier will accidentally give you a tank that does not have a siphon tube, or the siphon may be damaged/disconnected and the supplier is not aware. In either case, there is a simple way to validate if there is an issue with the siphon:

The Tip Test!

With either a damaged or missing Siphon, the Liquid CO2 will not be accessible. If you have a second person (carefully) hold the tank upside down and then check the discharge from the device, you should see the expected foggy spray. By turning the tank upside down, you are forcing the Liquid CO2 in the tank to sit directly on the valve, allowing it to be directly extracted. If the Tip Test produces the expected discharge, in most cases the supplier will just swap the tank out for you.

No SiphonValve onlyextracts gasTank UpsideDownValve canextract Liquid

If the Tip test doesn’t produce the expected discharge, there is one last possible cause…

Dry Ice Formation.

Under certain, uncommon, conditions the Liquid CO2 in the tank can flash freeze into dry ice. In the rare event this happens, typically you will be dispensing gas from the device and suddenly the stream will slow or stop completely. It can also present with the bottom of the tank feeling especially cold to the touch, or forming frost of condensation. If this happens, stop dispensing gas and close the tank valve. Normally, letting the tank sit in a warm room for a couple of hours will resolve this, however you may need to return it to the supplier for a fresh tank.

Dry Ice PlugIn SiphonDry Ice inBottom ofTank

Conclusion

I hope this has helped demystify why CO2 is used for Localized Cryotherapy, and helped understand some of the nuance around CO2! If you have any questions I would love to hear from you!

I am actively involved with America Cryo in sales as an Equine Specialist, as well as a Master Trainer for all of America Cryo's Equine devices. While the information and opinions expressed here are my own, I do want to acknowledge the potential bias that may come with this. Interested in learning more? I would love to hear from you!